Avinash Thakur et al INDUCTION PROTOCOLS FOR NEURONAL TRANSFORMATION OF GLOBOSE BASAL STEM CELLS

Objective(s): Consistent efforts with increasing optimistic outcome are being made to identify a candidate (neuronprogenitor cell) for a reliable therapeutic intervention of CNS injury. In this venture, recent years witnessed an increasing bang on the olfactory epithelium for suitable neuripotent stem cells. In this research work we aim at exploring the cultural characteristics and standardizing induction media for neuronal transformation of the potential multipotent Globose basal stem cells (GBC) residing in the basal compartment of the olfactory epithelium of rat olfactory mucosa. Methodology: GBC were isolated in pure form from olfactory mucosa of albino wistar rats using GBC-III monoclonal antibody and fluorescence activated cell sorting (FACS). Two different induction protocols were defined for the transformation of GBC into functional neurons. Results: GBC transformed into neurons and astrocytes in both the induction media. The neurons and astrocytes hence formed were immunohistologically characterized. Conclusion: This research work conclusively elucidates the cultural characteristics of a novel multipotent candidate (GBC) which can be induced into functional neurons using specific external niche and can prove to be beneficial for further research in neuro-regenerative therapies.